Isooctyl acetate was synthesized with ethyl acetate and iso-octyl alcohol as raw materials, cation exchange resin NKC-9 as catalyst and 4A zeolite as dealcohol adsorbent. The effects of molar ratio of ethyl acetate to isooctyl alcohol, catalyst dosage, reaction temperature and time on the transesterification were examined. The results showed that the optimum reaction conditions were obtained as the molar ratio of ethyl acetate to isooctyl alcohol at 3.0:1, catalyst dosage 6.0% (referring to total mass of ethyl acetate and isooctyl alcohol), reaction temperature ≤98℃, and reaction time 4.0 h. Under the conditions, the content of isooctyl acetate in the reaction solution could achieve 60.51%.