In this study, lactic acid fermentation by R.oryzae was investigated using the two different fermentation strategies of one-step fermentation (OSF) and conventional fermentation (CF). The lactate production medium, which included different nitrogen sources, different peptone concentration, was inoculated directly from the spore suspension. The different cell density was controlled in the fermentation. Compared to CF, OSF reduced the demurrage of the production process and increased the production of lactic acid. However, the unit biomass utilization rate was significantly lower than during CF. Based on analysis of μ, qs and qp, a novel modified OSF strategy was proposed. This strategy aimed to achieve a high final concentration of lactic acid, and a high unit biomass utilization rate by R.oryzae. In this strategy, the maximum lactic acid concentration and productivity of the lactic acid production stage reached 158 g/l and 5.45 g/(l·h), which were177 % and 366 % higher, respectively, than the best results from CF. Importantly, the qp and yield did not decrease. This strategy is a convenient and economical method for L-lactic acid fermentation by R.oryzae.
HU Jia-huan ZHANG Gu-han FU Yong-qian ZHU Ting-ting
. High-efficiency L-lactic acid production by Rhizopus oryzae using a novel one-step fermentation strategy[J]. The Chinese Journal of Process Engineering, 2017
, 17(2)
: 375
-381
.
DOI: 10.12034/j.issn.1009-606X.216303
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