True Bone Ceramic/ type I collagen (TBC/CoL) composite scaffolds was prepared, and its physicochemical properties and cell compatibility were evaluated. The TBC and CoL was mixed with different ratio and collagen crosslinked by glutaraldehyde. The heterogeneous mixture was freeze-dried and the dehydrated state was obtained as the scaffold. The effects of different materials and cross-linking of collagen on the scaffold properties were investigated. The physical and chemical properties of the scaffolds were analyzed by scanning electron microscopy (SEM), X-ray diffraction (XRD), fourier transform infrared spectroscopy (FIRT) and differential scanning calorimetry (DSC-TGA). The proliferation of the cells on the scaffold was evaluated by the CCK-8 method. SEM results show that the prepared TBC/CoL scaffolds have a porous structure with an average porosity of 93.5% and a Young's modulus of 4.36 ± 0.21 MPa. XRD and FTIR spectra indicated that TBC in the composite scaffolds were chemically adsorbed on the CoL matrix and the CoL structure in the matrix remained intact. MC3T3-E1 cells were cultured on the scaffold. After 24 h of culture, the cells grew well on the scaffold. CCK-8 assay showed that the proliferation of cells on the scaffold was not significantly different from the control group (p>0.05). The results showed that the scaffold prepared by True Bone Ceramic and type I collagen had good porosity, good mechanical strength, good cell compatibility and cell proliferation.
LEI Xiong-xin LI Sai-na KANG Ji-yao PENG Gong-ze ZHANG Gui-feng
. Preparation and Evaluation of True Bone Ceramic/Type I Collagen Composite Bone Repair Materials[J]. The Chinese Journal of Process Engineering, 2017
, 17(2)
: 389
-394
.
DOI: 10.12034/j.issn.1009-606X.216334
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