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Biochemical Engineering

Relationship between Gene Expression of ADH Isozymes and Asymmetric Reduction of 2-Octanone with Saccharomyces cerevisiae

  • xian'ai Shi Haihong LIN Xiaoping WANG Shiyin FANG
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  • 1. College of Biological Science and Technology, Fuzhou University, Fuzhou, Fujian 350108, China; 
    2. Institute of Pharmarceutical Biotechnology and Bioengineering, Fuzhou University, Fuzhou, Fujian 350108, China; 
    3. Fujian Key Lab of Medical Instrument and Pharmarceutical Technology, Fuzhou University, Fuzhou, Fujian 350108, China

Received date: 2016-11-21

  Revised date: 2017-01-04

  Online published: 2017-08-16

Abstract

In the water/n-dodecane biphasic reaction system, the influence of organic solvent treatment on the gene level of isozymes of alcohol dehydrogenase (ADH) 1~3 was investigated to probe their correlation in asymmetric reduction of the substrate 2-octanone by Saccharomyces cerevisiae type II. The results showed that after treatment with organic media, the relative level of isozymes of alcohol dehydrogenase (ADH) varied, along with the different properties shown in asymmetric reduction of 2-octanone treatmented by Saccharomyces cerevisiae, the gene level of ADH 1 was severely inhibited by toluene and 2-octanol, but it was not obviously influenced by n-dodecane, n-octane and n-hexane, while substantially enhanced by 2-octanone. However, the gene level of ADH 2 was highly increased by toluene and 2-octanol, but inhibited by n-dodecane, n-octane, n-hexane, and 2-octanone. Furthermore, the inhibition of ADH 1 gene would reduce the catalytic activity, and inhibition of ADH 2 gene would improve the enantiomeric selectivity, while the gene level of ADH 3 would not influence both the activity and selectivity. The isozyme ADH 1 which has good enantio selectivity is the key oxidoreductase for catalyzing asymmetric reduction of 2-ocatnone by Saccharomyces cerevisiae type II, and the isozyme ADH 2 is of poor enantio selectivity while it conducts the same reaction.

Cite this article

xian'ai Shi Haihong LIN Xiaoping WANG Shiyin FANG . Relationship between Gene Expression of ADH Isozymes and Asymmetric Reduction of 2-Octanone with Saccharomyces cerevisiae[J]. The Chinese Journal of Process Engineering, 2017 , 17(4) : 827 -833 . DOI: 10.12034/j.issn.1009-606X.216350

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