Different organic media were used to induce changes of cell membrane properties, i.e. the membrane permeability, mobility and integrity. The effect of organic media treatment on the asymmetric reduction of 2-octanone catalyzed by Saccharomyces cerevisiae cells was investigated. The results showed that the reaction yield was significantly decreased when the leakage values of nucleic acid and protein reached 0.057 and 0.046 respectively, or the propidium iodide (PI) uptake factor reached 1.157. When the anisotropy value of cell membrane labeled with diphenylhexatriene (DPH) reached 111.7% of the control group, the cells lost the ability of catalyzing asymmetric reduction. When the ratio of cell with damaged membrane integrity reached 41.8%, the reaction yield was significantly reduced. In the premise of maintaining the integrity of cell membrane, cell membrane permeability and mobility in the appropriate range make Saccharomyces cerevisiae cells show good performance on catalyzing 2-octanone asymmetric reduction reaction.
Feng LI Shiyin FANG Xianai SHI
. Effects of Organic Media Treatment Saccharomyces cerevisiae on Its Catalytic Performance to Asymmetric Reduction Reaction[J]. The Chinese Journal of Process Engineering, 2017
, 17(6)
: 1281
-1286
.
DOI: 10.12034/j.issn.1009-606X.217217
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