Based on the syringe pumps and cooperated with the developed software, an adjustable gradient mobile phase control system was developed, furthermore the system was connected with quartz crystal microbalance (QCM). Through the gradient adjustion of salt concentration or pH value, the real time adsorption and elution variations of bovine serum albumin (BSA) were investigated. The results showed that the flow error was 1%, the flow stability was 1%, and the gradient error was 1%, which met the standard of GB/T 26792-2011. With the increase of salt concentration or decrease of pH value, the BSA was eluted gradually. These results demonstrated that this system could regulate the gradient and simulate complicated solid?liquid interface environment accurately.
Cui SONG Jia GE Xiunan LI Qiang LI Chao CHEN Peng WANG Guanghui MA?
. Application of Adjustable Gradient Mobile Phase Control System on Biomacromolecule Characterization[J]. The Chinese Journal of Process Engineering, 2018
, 18(1)
: 171
-176
.
DOI: 10.12034/j.issn.1009-606X.217230
[1]Mereghetti P, Gabdoulline R R, Wade R C.Brownian dynamics simulation of protein solutions: structural and dynamical properties[J].Biophys J., 2010, 99(11):3782-3791
[2]Wlodarski T, Zagrovic B.Conformational selection and induced fit mechanism underlie specificity in noncovalent interactions with ubiquitin[J].Proc Natl Acad Sci U S A., 2009, 106(46):19346-19351
[3] Berg O G, von Hippel P H.Diffusion-controlled macromolecular interactions [J]. Annu Rev Biophys Biophys Chem., 1985, 14: 131-160.
[4] David B, Philipp T, Jeroen B, Christian O.Stabilization of Protein-Protein Interactions in chemical biologyand drug discovery [J].Progress in Biophysics and Molecular Biology., 2015, 119: 10-19.
[5] Robinson G W, Cho C H.Role of hydration water in protein unfolding [J]. Biophys. J., 1999, 77: 3311–3318.
[6] Fenimore P W, Frauenfelder H, Mcmahon B H, et al.Slaving: solvent fluctuations dominate protein dynamics and functions [J]. Proc. Natl. Acad. Sci. U. S. A., 2002, 99: 16047–16051.
[7] Pace C N, Laurents D V, Erickson B E.Urea denaturation of barnase: pH dependence and characterization of the unfolded state [J]. Biochemistry, 1992, 31: 2728–2734.
[8] Song W, Chen H.Protein adsorption on materials surfaces with nano-topography [J]. Chinese Science Bulletin, 2007, 52: 3169-3173.
[9] Sharma S, PoPat KC, Desai TA.Controlling Nonspecific Protein Interactions in Silicon Biomicrosystems with Nanostructured Poly(ethylene glycol)Films [J]. Langmuir, 2002, 18: 8728-8731.
[10] Cristina F, Gabriela C, Conxita S, Christine V.Protein–nanoparticle interactions evaluation by immunomethods: Surfactants can disturb quantitative determinations [J].European Journal of Pharmaceutics and Biopharmaceutics, 2015, 94:284-290
[11] Zhang H Y, Yang L Q, Zhou B J, et al.Investigation of biological cell–protein interactions using SPR sensorthrough laser scanning confocal imaging–surface plasmon resonancesystem [J]. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 2014, 121:381-386.
[12]张志荣，董尔丹，吴镭等.生物大分子药物递送系统研究现状与前沿方向[J].中国基础科学, 2014, 16(5):3-9
[13]Zhang Z R, Dong E D, Wu L, et al.Progress and Future Development on Efficient in Vivo Delivery System of Biomacromolecular Drugs[J].China Basic Science, 2014, 16(5):3-9
[14] O’Sullivan C K, Guilbault G G.Commercial quartz crystal microbalances – theory and applications [J]. Biosensors & Bioelectronics, 1999, 14: 663-670
[15] P?ibyl J, Skládal P.Quartz crystal biosensor for detection of sugars and glycated hemoglobin [J]. Analytica Chimica Acta, 2005, 530: 75-84.
[16] Feiler A A, Sahlholm A, Sandberg T, Caldwell K D.Adsorption and viscoelastic properties of fractionated mucin (BSM) and bovine serum albumin (BSA) studied with quartz crystal microbalance (QCM-D) [J]. Journal of Colloid and Interface Science, 2007, 315: 475-481.
[17]Liu T, Tang J A, Han M M, et al.Surface modification of nanogold particles in DNA detection with quartz crystal microbalance[J].Chinese Science Bulletin, 2003, 48(9):873-875
[18] JJG024-1996, 高效液相色谱仪检定规程.
[19] GB/T 26792-2011, 高效液相色谱仪.
