A two-step chromatographic method of purifying transferrin (Tf) from Cohn fraction IV, a byproduct of the Cohn plasma fractionation process, was established. After diluting, centrifugation and filtration, the Cohn fraction IV was firstly purified by Capto DEAE ion-exchange chromatography, then further purified by hydrophobic interaction chromatography. The characterization of purified Tf product was analyzed by SDS?PAGE, HPLC and ELISA. The results indicated that the purity of Tf was greater than 99% and the yield was 79%. The circular dichroism spectrum proved that the secondary structure of the purified Tf product have not changed, and iron binding ability experiment proved that the purified Tf product can bind two iron ions.
Jinlong KONG Can QUAN Qidi WANG Jian LUO Junbo ZHOU Yongyue SUN Zenglan LI
. Purification of Human Serum Transferrin Reference Material[J]. The Chinese Journal of Process Engineering, 2018
, 18(2)
: 387
-392
.
DOI: 10.12034/j.issn.1009-606X.217300
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