Two algal species Chlorella vulgaris and Anabaena vasriabilis were chosen as typical energy microalgae to investigate the surface characteristics of microaglae. The surface potential and hydrophobicity was examined based on the extend XDLVO theory. According to the Zeta potential, hexadecyl trimethyl ammonium bromide (C16TAB) was used as cationic surfactant for flotation. The results showed that both two strains carried with negative charge, and free energy of cohesion for C. vulgaris was 1.21 mJ/m2, and ?55.85 mJ/m2 for A. varsriabilis, which meant hydrophilicity and hydrophobicity respectively. A. varsriabilis performed better harvesting rate than C. vulgaris. The maximums concentration factor (12.45 for C. vulgaris and 1.3 for A. varsriabilis) toped at the pH which charged most negatively (pH=7 for C. vulgaris and pH=9 for A. varsriabilis). Nevertheless, harvesting rate toped at pH=10, which meant it could not reach the maximum with the concentration factor in the same condition, may owing to the drainage of the tonoplast. Meanwhile, after adding 80 mg/L C16TAB into the algal solution, the hydrophobicity rate for C. vulgaris raised from 19% to 64%, and the harvesting rate increased by 67.38%.
Zhou SHEN Hao WEN Xiangying REN Jun LIU Liwei YANG Yanpeng LI
. Influences of Surface Characteristics of Energy Microalgae on Its Harvesting Performance by Air Flotation[J]. The Chinese Journal of Process Engineering, 2018
, 18(2)
: 441
-446
.
DOI: 10.12034/j.issn.1009-606X.217271
[1] Seo Y H, Park D, Oh Y K, et al.Harvesting of microalgae cell using oxidized dye wastewater[J].Bioresource Technology, 2015, (192):802-806
[2]Kenny P, Flynn K J.In silico optimization for production of biomass and biofuel feedstocks from microalgae[J].Journal of Applied Phycology, 2015, 27(1):33-48
[3]Koberg M.Optimization of bio-diesel production from oils,cooking oils,microalgae,and castor and jatropha seeds: probing various heating sources and catalysts[J].Energy & Environmental Science, 2012, 5(6):7460-7469
[4]Spiden E M, Yap B H J, Hill D R A, et al.Quantitative evaluation of the ease of rupture of industrially promising microalgae by high pressure homogenization[J].Bioresource Technology, 2013, 140(3):165-171
[5]Milledge J J, Heaven S.A review of the harvesting of micro-algae for biofuel production[J].Reviews in Environmental Science and BioTechnology, 2013, 12(2):165-178
[6]Wu Z, Zhu Y, Huang W, et al.Evaluation of flocculation induced by pH increase for harvesting microalgae and reuse of flocculated medium[J].Bioresource technology, 2012, 110(2):496-502
[7]Prochazkova G, Safarik I, Branyik T.Harvesting microalgae with microwave synthesized magnetic microparticles[J].Bioresource Technology, 2013, 130(1):472-477
[8]Coward T, Lee J G M, Caldwell G S.Development of a foam flotation system for harvesting microalgae biomass[J].Algal Research, 2013, 2(2):135-144
[9]Ozkan A, Berberoglu H.Physico-chemical surface properties of microalgae[J].Colloids & Surfaces B Biointerfaces, 2013, 112(3):287-293
[10]Garg S, Wang L, Schenk P M.Effective harvesting of low surface-hydrophobicity microalgae by froth flotation[J].Bioresource Technology, 2014, 159(2):437-441
[11] Fowkes F M.Contact angle, wettability, and adhesion[M]// Contact angle, wettability and adhesion :. American Chemical Society, 1964:888-892.
[12]Rosenberg M, Gutnick D, Rosenberg E.Adherence of bacteria to hydrocarbons: A simple method for measuring cell-surface hydrophobicity[J].Fems Microbiology Letters, 1980, 9(1):29-33
[13]陈家长, 王菁, 裘丽萍, 等.对鱼腥藻和普通小球藻生长竞争的影响[J].生态环境学报, 2014, 42(2):316-320
[14]欧阳峥嵘, 温小斌, 耿亚红, 等.光照强度、温度、、盐度对小球藻光合作用的影响[J].植物科学学报, 2010, 28(1):49-55
[15]Eroglu, Melis, Anastasios.Extracellular terpenoid hydrocarbon extraction and quantitation from the green microalgae Botryococcus braunii varShowa[J].Bioresource Technology, 2010, 101(7):2359-2366
[16]Callow M E, Callow J A, Ista L K, et al.Use of self-assembled monolayers of different wettabilities to study surface selection and primary adhesion processes of green algal (Enteromorpha) zoospores[J].Applied & Environmental Microbiology, 2000, 66(8):3249-3254
[17]A.F. Toussaint,PELuner,The wetting properties of grafted cellulose films[J].Journal of Adhesion Science and Technology, 1993, 7(6):635-648
[18]孟顺龙, 王菁, 裘丽萍, 等.氮磷质量浓度对普通小球藻和鱼腥藻生长竞争的影响[J].生态环境学报, 2015, 5(4):658-664
[19]Pi?eres J, Barraza J.Energy barrier of aggregates coal particle–bubble through the extended DLVO theory[J].International Journal of Mineral Processing, 2011, 100(1):14-20
[20]Coward T, Lee J G M, Caldwell G S.Harvesting microalgae by CTAB-aided foam flotation increases lipid recovery and improves fatty acid methyl ester characteristics[J].Biomass & Bioenergy, 2014, 67(9):354-362
[21] Zhao Y, Li Y P, Huang J, et al.Rebound and attachment involving single bubble and particle in the separation of plastics by froth flotation[J].Separation & Purification Technology, 2015, (144):123-132