Propionibacterium freudenreichii is regarded as a GRAS (generally recognized as safe) strain, and has been successfully applied to industrial anaerobic production of deoxyadenosylcobalamin, known as the natural structure of vitamin B12. Generally, due to the unstable of deoxyadenosylcobalamin, it needs to be converted to cyanocobalamin by cyanidation during the subsequent extraction and purification steps. Hydroxycobalamin is another form of vitamin B12 and widely used in the treatment of neurological diseases and cyanide poisoning. For the industrial production of hydroxycobalamin, cyanocobalamin is usually used as the raw material and then deacylated to form hydroxycobalamin. According to this principle, the production of hydroxycobalamin involves multiple chemical reactions, including cyanidation and deacylation, along with the high cost, environmental pollution, and hazardous conditions to the operators. To avoid these disadvantages, the photolysis production of hydroxycobalamin directly from deoxyadenosylcobalamin was proposed. In this work, the adsorption and extraction of hydroxycobalamin in the deoxyadenosylcobalamin photolysis solution were studied. The adsorption properties of 8 kinds of different macroporous resins on hydroxycobalamin were compared, and the effects of pH and ethanol concentration on hydroxycobalamin adsorption and desorption were discussed. The loading and elution velocity were optimized to improve the hydroxycobalamin yield. The results showed that LX-50B macroporous resin had the best adsorption performance for hydroxycobalamin in the static adsorption process. The adsorption capacity of LX-50B macroporous resin reached 203.87 mg/g at pH 6.0. For hydroxycobalamin eluent, 60% ethanol solution with pH 2.0 was the best choice. The optimal loading velocity and elution velocity were 2 and 0.5 BV(bed volume)/h, respectively. Under the above conditions, the concentration of hydroxycobalamin in the elution was 1863.32 mg/L, improved by 35 times compared with the original photolysis solution, while the purity of hydroxycobalamin increased from 0.38% to 15.73%, with an acceptable extraction yield of 92.46%, which achieved to a good extraction and preliminary purification result.
Jun YANG Ziqiang WANG Yunshan WANG Wanzhong ZHANG Zhiguo SU
. Extraction of hydroxycobalamin with the method of macroporous resin adsorption[J]. The Chinese Journal of Process Engineering, 2019
, 19(4)
: 728
-734
.
DOI: 10.12034/j.issn.1009-606X.218311
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