A method for the determination of molecular and molecular weight distribution of alginate by gel chromatography and conventional detector was proposed. Alginates in a wide range of viscosities extracted from two dominated raw materials—Lessonia nigrescence and Lessonia trabeculata were taken as the samples. The relative molecular weight of alginate was determined by high-performance gel permeation chromatography (GPC) combined with refractive index detector (RID) with pullulan served as a standard. The corresponding relations of relative weight average molecular weight ( ), relative number average molecular weight ( ) measured by GPC–RID and absolute weight average molecular weight ( ), absolute number average molecular weight ( ) measured by multi-angle laser scattering (GPC–MALLS) were obtained by least square method. The results showed that there was a good linear correlation between the relative molecular weight of alginate determined by GPC–RID and the absolute molecular weight of alginate determined by GPC–MALLS. The correlation index was more than 0.9. The relative error between the absolute weight average molecular weight determined by the established GPC–RID method and the absolute weight average molecular determined by GPC–MALLS was less than ?12%. The relative error between the absolute number average molecular weight determined by the established GPC–RID method and the absolute number average molecular determined by GPC–MALLS was less than ?12%. The polydispersity index calculated by the established GPC–RID method was less than ?12%, compared with that obtained by GPC–MALLS method. The GPC–RID method for the determination of the relative molecular weight of alginate can be used to determinate the absolute molecular weight and the polydispersity index of alginate. It manifested that it was feasible and economical to use the GPC–RID method provided in this work for the determination absolute of the absolute weight molecular weight, absolute number molecular weight and polydispersity index of alginate. The method can save the investment on the instrument and reduce the cost of determination the molecular weight of alginate.
Ruixin ZHANG Zhihui WANG Wei CONG Fengji HUI Bin WANG
. Molecular weight and molecular weight distribution of alginate determination by gel permeation chromatography[J]. The Chinese Journal of Process Engineering, 2020
, 20(1)
: 99
-107
.
DOI: 10.12034/j.issn.1009-606X.219177
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