n order to realize the efficient separation of Fe2+ in acidic media, hydrogen peroxide oxidation method is often used in industry to transform Fe2+ into Fe3+ with lower solubility to realize the precipitation of iron. Due to the low utilization rate of hydrogen peroxide and poor economy, it is urgent to develop a new Fe2+ low-cost and high-efficiency oxidation method. Based on the principle that reactive oxygen species can be generated during the microbubbles bursting, the microbubble enhanced Fe2+ oxidation technology had been developed in this study. The effect of the aeration head aperture, reaction temperature, and acid concentration on the oxidation efficiencies of Fe2+ and ?OH production were studied. The oxidation efficiency of Fe2+ can reach to 99% within 30 min under the optimized conditions (90℃, 30wt% H3PO4, 0.22 μm aeration head aperture). The oxidation effect of microbubble enhanced technology was comparable to the current H2O2 oxidation, and greatly reduced the economic cost of the process. Moreover, the mechanism of Fe2+ oxidation enhanced by microbubbles was studied in this work, and the main reactive oxygen species generated by microbubbles bursting were determined to be hydroxyl radicals, and the influences of aeration head aperture, reaction temperature and acid concentration on the generation of hydroxyl radicals were studied, so as to obtain the regulation rules of hydroxyl radicals in acidic media.