Nowadays, the production and combustion of fossil fuels make the emission of particulate pollutants increasing day by day. Researchers are actively developing new high efficiency dust removal separator to reduce the emission of pollutants. The studies on the separation technology of single cyclone separator and single particle bed filter are extensive but on the coupling separator of cyclone separator and particle bed are scarce. Therefore, the study of the cyclone particle bed coupling separator has broad application prospect. In order to investigate the influences of the inlet dust concentration on the pressure drop and the dust removal efficiency of a new cyclone-granular bed coupling separator, a large scale cold model experiment was carried out. The results showed that with an increase of the inlet dust concentration, the pressure drop of the separator tenders large and the speed of pressure drop increased rapidly when reaching an equilibrium state. The reason is that the inlet dust concentration increases, the dust in the built-in particle bed increases, the bed porosity decreases and the pressure drop increases. With the decrease of the inlet dust concentration, the separation efficiency of the separator increased. The dust content in the particle bed of the separator was also related to the regeneration efficiency. As the concentration of the regeneration dust source decreased, the time duration of the pressure drop reaching equilibrium became short. Furthermore, based on the experimental data, the empirical formula of separator pressure drop and regeneration dust source concentration was given. When the concentration of regeneration dust source reduced from 58.18% to 23.67%, the pressure drop reduced from 1.5 kPa to 1.2 kPa. With the decrease of the concentration of the regeneration dust source, the dust removal efficiency of the separator decreased slightly. The influence of inlet dust concentration and the regeneration dust source concentration on the pressure drop of separator coincided with each other, while the effect on the separator efficiency was different.