Foot-and-mouth disease vaccine is a veterinary vaccine, which plays an important role in the development of animal husbandry. However, there are some problems of foot-and-mouth disease vaccines such as poor antigen thermal stability and easy inactivation of antigen. At present, researchers have modified amino acids at the junction of foot-and-mouth disease virus pentamer as one of the strategies to improve the thermal stability of foot-and-mouth disease virus vaccine. This method is complicated to operate and needs to be designed separately for different serotypes of viral capsids. Therefore, this research proposes a strategy that combines antigen stabilization with adjuvant design, combing the oli-in-water emulsion with aluminum hydroxide particles to construct a particle-stabilized emulsion (Pickering emulsion), which would improve the thermal stability of the foot-and-mouth disease vaccine. In this work, spherical, rod-shaped, and flaky aluminum hydroxide particles were prepared by hydrothermal method. The prepared aluminum hydroxide particles had good dispersion, uniform particle size distribution and high crystallinity. Due to the rough surface of Pickering emulsions, antigens were absorbed on the surface and cracks of particles. On this basis, squalene was used as oil phase to prepare Al-Pickering emulsion with uniform size. Subsequently, differential scanning fluorimetry was used to evaluate the thermal stability of inactivated foot-and-mouth disease virus (iFMDV) combined with Al-Pickering emulsion. The results showed that each group of Al-Pickering emulsions improved the thermal stability of iFMDV. The rod-Pickering emulsion had the best promotion effect, and the pyrolysis temperature of iFMDV increased by 1.7?C. The adjuvant effect of Al-Pickering was evaluated by animal experiment. Animal experiment results showed that Al-Pickering emulsion effectively activated the titer of iFMDV specific antibody IgG in serum. In summary, Al-Pickering enhanced the humoral responses and stabilized iFMDV after loading. In addition, they are easily prepared and low-cost. It provides a new idea for the research of adjuvants to improve the thermal stability of vaccines.