At present, the multi-hole plates with uniform hole distribution structure are widely used in the process industry to limit the flow and reduce the pressure, vibration and noise because of low manufacturing cost, simple structure, easy processing, easy installation and maintenance. In order to further improve the flow stability and reduce the flow noise, multi-hole plates of non-uniform hole distribution with different hole spacing, hole number, and hole diameter are designed with the equivalent opening diameter unchanged. Based on the numerical results, the velocity, pressure, reflux characteristics, jet convergence and flow development in the pipeline with different multi-hole plates are compared in detail to analyze the flow field characteristics. Moreover, the noise of multi-hole plates is numerically calculated by the acoustic analogy model. The observation points are set on the centerline and the section perpendicular to the flow direction to compare the spectrum characteristics and the overall sound pressure level. The numerical results show that the decrease of hole spacing with equal difference, the increase of hole number on the plate edge, and the increase of hole diameter without reducing the hole number can all effectively improve the flow stability and reduce the noise level of multi-hole plates without influencing the pressure drop ability compared with the general multi-hole plate with uniform hole distribution, and the maximum decrease of noise level is 5.62, 6.10 and 7.00 dB respectively.