Oxytetracycline is widely used in animal husbandry and aquaculture because it can effectively inactivate Gram-positive and Gram-negative bacteria. However, 50%~80% of oxytetracycline is not fully absorbed and utilized, and enters the environmental units such as surface water, groundwater, and soil through sewage discharge, surface runoff, and landfill leachate in the form of livestock parent or metabolite. Therefore, it is urgent to take fast and efficient means to deal with oxytetracycline pollution. Photocatalytic technology is widely used in organic compounds due to its low energy consumption, high removal rate, low cost, and ability to convert organic pollutants into harmless small molecular organic compounds. Among the existing photocatalysts, tungstate photocatalysts stand out due to their wide absorption wavelength range, narrow band gap, and high stability. The natural semiconductor mineral wolframite was used as a photocatalyst for the degradation of oxytetracycline in wastewater. The effects of solid-liquid ratio, light intensity, and initial concentration on the degradation of oxytetracycline were investigated while the degradation mechanism and pathway were analyzed. The results showed that wolframite had a promising degradation effect on oxytetracycline under visible light, and the removal efficiency of oxytetracycline could reach 94.3% at 120 min under the conditions of concentration of 15 mg/L, pH=4.5, the solid-liquid ratio of 1.0 g/L and light intensity of 60 W; The degradation of oxytetracycline by wolframite is in accordance with the primary kinetic reaction law. The results of the quenching experiment and electron paramagnetic spectroscopy showed that hydroxyl radical (?OH) and superoxide anion radical (?O2-) were the main active oxidizing species causing oxytetracycline degradation, which degraded 20.4% and 10% oxytetracycline at 120 min, respectively. The results of liquid chromatography-mass spectrometry showed that oxytetracycline was degraded into intermediates through hydroxylation, demethylation, and decarbonylation, which was beneficial to the industrial application of wolframite in antibiotic treatment.