The obstacles in the microchannel have a significant impact on the pressure drop and bubble shape of gas-liquid two-phase flow. In this work, experimental and numerical simulation methods are used to explore the effect of obstacles in the channel on the movement characteristics of nitrogen/water gas-liquid two-phase flow in the microchannel. The variations of pressure drop and bubble length under different gas and liquid flow rates in microchannel with obstacles are analyzed. The results show that the pressure drop in the obstacle microchannel is higher than that in the barrier free microchannel, and the maximum pressure drop occurs with the obstacle in the center. Through numerical simulation analysis, this is due to the vortex generated after the obstacle, and the pressure drop is positively related to the vortex length. The bubble length of the obstacle microchannel changes within 25% compared to the barrier free channel, and the bubble length becomes shorter as the obstacle approaches the center. Under different flowing conditions, there are three phenomena when bubbles pass through obstacles, including retraction without rupture, retraction with rupture, and direct rupture without retraction. The retraction lengths increase with capillary number increasing in retraction without rupture. The retraction lengths reduce with capillary number increasing in retraction with rupture. When the retraction length decreases to 0, it become a direct fracture without retraction. The variation range of retraction length gradually increases as the obstacle approaches the center. When passing through all obstacles, different breaking and merging laws are displayed under different working conditions. By numerical simulation, different vortex lengths after the obstacle result in different pressure drops, and there exists sever change at the moment of bubble rupture. When passing through the obstacle, the change of bubble shape is affected by the change of liquid phase velocity around it, different velocities in the sub channels on both sides of the obstacle determine the different rules of the two sub bubbles after passing through the obstacle.