Tengchong zinc oxide ore was studied for the technological problems of fine mineral dissemination and high content slime. The results show that the grade of zinc in the ore is 6.65% and the iron 12.31%. The oxidation date of zinc is up to 92.83%, calamine is the main existing form of the zinc and iron mainly in the form of magnetite. After comparative experiments of multiple trials processes, it is determined that the processes of washing are used in advance for removing the particles less than 37 μm, and the sand grinding and magnetic flotation separation are followed. The optimum particle fineness of 91.74% less than 75 μm is fixed, and the current of low intensity magnetic separation is 4 A. The roughing reagent system consists of sodium carbonate 2 kg/t, sodium hexametaphosphate 2 kg/t, sodium sulfide 13 kg/t and KPR 2 kg/t. By the closed circuit experiments, the zinc concentrate of zinc grade 27.89% and the recovery rate of 75.26%, and the iron concentrate of iron grade 61.25% and the recovery rate of 51.65% are obtained. And the functional mechanism of KPR with calamine is also analyzed based on the Zeta potential measurement.