Chicken NDV-AIV bigeminal vaccine white oil emulsion adjuvant was prepared by high speed stirring (HS) and rapid membrane emulsification (ME), respectively, the relationship between emulsion uniformity and inflammation and immunological effect injection sites was studied. The results showed that emulsion particle size of both kinds of vaccine were about 1.0 mm. Polydispesity index (PDI) value of ME vaccine was 0.036, and that of HS vaccine 0.279. ME vaccine showed better uniformity. The two kinds of vaccine had no significant difference in HI and IgG antibody level. Inflammatory factor IL-1b, IL-6, IL-17 and TNF-a gene expression levels of HS vaccine were up to 45, 7, 25 and 80 pg/mL, which were significantly higher than that of ME vaccine (the expression quantities were all less than 5 pg/mL). The white oil emulsion adjuvant with uniform size could induce enough immune protection, and cause milder inflammation.