Abstract: The carbon and nitrogen sources during the bioconversion from L-phenylalanine to 2-phenylethanol via Saccharomyces cerevisiae were optimized. In a 5-L fermentor, final 2-phenylethanol concentration of 4.39 g/L, space-time yield of 0.314 g/(L×h), molar yield of 0.8 and residual L-phenylalanine concentration of 0.634 g/L were achieved when respiratory quotient was controlled at 1.1±0.1 by feeding glucose, and 1 g/L urea and 8 g/L L-phenylalanine were selected as nitrogen source and precursor respectively.

Key words: 2-phenylethanol, Saccharomyces cerevisiae, respiratory quotient, fed-batch culture, optimization

摘要： 对酿酒酵母转化生成2-苯乙醇过程的碳氮源进行优化，选择葡萄糖为碳源，1 g/L脲为氮源，8 g/L L-苯丙氨酸为前体，以呼吸商达1.1±0.1为补糖控制依据，在5 L发酵罐水平建立分批补料工艺，2-苯乙醇终浓度达4.39 g/L，生成速率达0.314 g/(L×h)，L-苯丙氨酸摩尔转化率为0.8，L-苯丙氨酸残留浓度仅为0.634 g/L.

关键词: 2-苯乙醇, 酿酒酵母, 呼吸商, 分批补料培养, 优化