以二乙烯基苯为单体，采用微孔膜乳化法制备了尺寸均一的水包油乳液，在75℃下悬浮聚合20 h，制得均一的聚二乙烯基苯微球填料，粒径为14.2 mm，粒径分布系数Rspan=0.71，孔径为65 nm，比表面积为160 m2/g. 通过高压匀浆法将合成的微球介质高效装填不锈钢液相色谱柱(f4.6 mm′250 mm），使柱效达11000塔板数. 利用装填的色谱柱进行多肽药物奥曲肽的分离纯化，奥曲肽从固相合成粗品中快速有效分离，纯度由42.89%提高到99.99%.

Polydivinylbenzene (pDVB) microspheres with the particle size of 14.2 mm were prepared with divinylbenzene as monomer by membrane emulsification and suspension polymerization at 75℃ for 20 h. The prepared pDVB microspheres had uniform particle size (Rspan=0.71), mean pore size of 65 nm and specific surface area of 160 m2/g. The pDVB microspheres were packed into f4.6 mm′250 mm stainless steel column by slurry packing method, and tested in separation of peptide drug by high performance liquid chromatography. Afterword, a very fast separation process of octreotide from synthetic peptide crude mixtures on pDVB reversed phase was carried out. Fourier-transformed infrared resonance (FT-IR) spectroscopy, and reversed-phase HPLC combined with electrospray ionisation mass spectrometry (ESI-MS) were used for analysis of resultant peptides. Under the optimized conditions, the octreotide was purified from 42.89% to 99.99%.