从8种树脂中筛选出能将g-氨基丁酸与谷氨酸钠分离的最佳树脂，考察了色谱柱高径比、洗脱流速、柱温对分离度和回收率的影响，用g-氨基丁酸发酵液上样，研究了发酵液的分离效果. 结果表明，树脂QY-HG01能将g-氨基丁酸与谷氨酸钠高效分离；在高径比25、流速2.5 mL/min、柱温50℃的条件下，g-氨基丁酸与谷氨酸钠的分离效果最好；用预处理的g-氨基丁酸发酵液上样，发酵液脱色率为84.9%，脱盐率为99.2%，g-氨基丁酸的纯度由24.9%提高到84.0%，回收率为89.3%.

Eight resins were tested systematically to select the best one for the chromatography separation of gamma-aminobutyric acid (GABA) and monosodium glutamate (MSG). Then the influence of the ratio of length to diameter of column, flow rate of mobile phase and column temperature on the separation efficiency of GABA and MSG were investigated in terms of resolution and recovery. Finally, the separation efficiency of fermentation broth was concretely studied. The results showed that the resin QY-HG01 separated GABA and MSG well. The best separation efficiency was achieved under the operating conditions of ratio of length to diameter 25, flow rate of mobile phase 2.5 mL/min and column temperature 50℃ through the single factor experiments. Injected with the pretreated fermentation broth, it was found that GABA and MSG were separated well. The decolorization rate of the fermentation broth was 84.9%, and the desalinization rate was 99.2%. The purity of GABA increased from 24.9% in the broth to 80.4% in the eluent with a recovery yield of 89.3%.