用7种不同性能的阴离子交换树脂吸附发酵液中丙酸，考察了发酵液酸化方式及pH值对吸附量的影响，分析了不同浓度H2SO4的解吸情况，比较了多柱串联组合层析提高丙酸收率的可行性. 结果表明，大孔弱碱性丙烯酸系阴离子交换树脂ZGD630对发酵液中的丙酸吸附量最高，采用H型阳离子交换树脂D001酸化工艺，当pH值由6.5降至3时，ZGD630树脂对发酵液中丙酸的静态吸附量从54.34 mg/g提高至110 mg/g，动态吸附量达152.5 mg/g. 用4 mol/L H2SO4进行解吸，丙酸浓度最高达81.8 g/L，为初始发酵液的2.12倍. 采用3柱串联组合层析时丙酸收率达62.15%，较单柱层析显著提高.

The adsorption capacity of 7 ion-exchange resins with different properties for propionic acid in the fermentation broth was investigated. The effects of fermentation broth acidification method and pH value on the resin adsorption capacity were studied. The elution processes with different concentrations of H2SO4 were analyzed. The feasibility of in series multi-column chromatography to increase the yield of propionic acid was discussed. The results showed that ZGD630, a macroporous weak-base anion exchange resin had the highest adsorption capacity for propionic acid. D001 H-type cation exchange resin acidization method was determined. When pH value decreased to 3 from 6.5, the static state adsorption capacity of ZGD630 increased from 54.34 mg/g to 110 mg/g dry beads, with a dynamic adsorption capacity of 152.5 mg/g. The highest propionic acid concentration during the elution process (4 mol/L H2SO4) reached 81.8 g/L, which was 2.12 times the original fermentation broth concentration. A recovery yield of 62.15% was obtained for propionic acid with in series three-column combined chromatography, a significant improvement compared to the one-column chromatography.