以疏水性草分枝杆菌M. ph为对照，选用从褐煤中筛选的细菌FML和酵母菌RH，研究微生物在肥煤原煤表面的吸附作用，分析pH值、矿浆浓度和细胞浓度等因素对吸附的影响. 结果表明，20 min内吸附即可达饱和，FML细菌与M. ph类似，吸附模式为单层吸附，RH细菌更接近多层吸附. 酵母菌RH在pH=4?6时吸附较稳定，吸附率达82%，吸附容量为3.2 mg/g. 微生物吸附后，煤表面性质发生了一定改变，RH更易吸附在精煤颗粒表面，可增强精煤的疏水性，FML则使原煤中矸石更亲水，因此可增大原煤中不同组分可浮性的差异，提高浮选效率.

The adsorption of the microorganisms on the surface of raw fat coal was researched, choosing bacteria FML and yeast RH which screened from lignite and hydrophobic Mycobacterium phlei (M. ph) for comparison. The influences of pH, slurry concentration and cell concentration on adsorption were analyzed. The results showed that the adsorption reached saturation in about 20 min. FML were found to follow the monolayer adsorption, similar to M. ph, while RH followed multilayer adsorption to a great degree. The adsorption of yeast RH was tested to be stable at pH=4?6 for the actual flotation system, and the adsorption rate and adsorption capacity were 82% and 3.2 mg/g, respectively. RH mainly adhered on the surface of clean coal particles and caused coal to be more hydrophobic, while the role of FML were mainly to make raw coal gangue more hydrophilic. The roles of both increased the raw coal floatability of different components and improve the flotation efficiency.