以六氟异丙醇为溶剂，采用喷雾静电纺丝技术将胶原(CoL)溶液与不同浓度的戊二醛(GA)共纺，通过原位交联技术获得GA?CoL共纺膜，对其进行了机械性能和生物学评价. 结果表明，GA浓度为9wt%时，GA?CoL共纺膜的纤维直径集中在450?750 nm，分布均匀，且CoL三螺旋结构保存完好，共纺膜断裂强度最高，为1.21?0.03 MPa，断裂延伸率最大，为6.48%，共纺膜接触角为45.4o. 共纺膜对细胞增殖的影响与对照组(纯CoL纺丝膜)没有显著差异(P>0.05).
Collagen bio-membrane possesses excellent biochemical characteristics, it has been widely used in the field of biomedicine. However, the existing membrane preparation techniques have series problems such as uneven membrane formation, low mechanical strength, and short degradation time after membrane formation. The modification method of compounding collagen with other polymer materials has the problems of immunological rejection and uncontrollable of degradation time. In this study, the collagen (CoL) membrane using spray electrospinning technique with hexafluoroisopropanol as the solvent was prepared. The synchronous crosslinking process was carried out during electrospinning with glutaraldehyde (GA) as crosslinking reagent. In order to optimize the suitable glutaraldehyde cross-linking concentration, the morphology, structure, mechanical properties, thermal denaturation temperature and the water contact angle (wettability) of the membrane were characterized by SEM, FT-IR, XRD, compressive test, DSC?TGA and contact-angle test respectively. Moreover, vitro experiments including co-culture assay, CCK-8 assay were executed to evaluate the biocompatibility of materials to cells. Series of tests and characterizations indicated that the optimal concentration of GA was 9wt%. The GA?CoL co-spun membrane obtained had uniform structure, in which the fiber diameter in the membrane was 450~750 nm. The FT-IR and XRD results reflected that the triple helix of collagen kept the native state. The mechanical properties test results indicated that the maximal strength of GA?CoL membrane was 1.21?0.03 MPa and the maximal elongation at break was 6.48%. The water contact angle of the spinning membrane was 45.4°, indicating the good hydrophilicity of the membrane. The biocompatibility test in vitro showed that MC3T3-E1 could adhere well on membrane and there was no significant difference in proliferation rate compared with the control group (P>0.05). Conclusively, the collagen-based biofilm prepared by spray electrospinning technique at a GA concentration of 9wt% had good physicochemical properties compared to other group of concentration, and this bio-membrane has good biocompatibility to MC3T3-E1 in vitro.
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