采用高速搅拌和快速膜乳化法制备鸡新流二联白油佐剂疫苗，考察了疫苗粒径均一性与疫苗引起的炎症反应和免疫学效应的关系. 结果表明，2种疫苗粒径约为1.0 mm，快速膜乳化疫苗(ME vaccine)的多分散系数(PDI)为0.036，高速搅拌疫苗(HS vaccine)的PDI值为0.279，前者粒径分布更均一. 两种疫苗的HI抗体和IgG抗体水平无显著差异. HS vaccine注射部位炎症因子IL-1b, IL-6, IL-17, TNF-a表达量最高分别为45, 7, 25和80 pg/mL，显著高于ME vaccine(均低于5 pg/mL). 均一白油佐剂疫苗在诱导有效免疫应答的同时，引起的炎症反应更轻.

Chicken NDV-AIV bigeminal vaccine white oil emulsion adjuvant was prepared by high speed stirring (HS) and rapid membrane emulsification (ME), respectively, the relationship between emulsion uniformity and inflammation and immunological effect injection sites was studied. The results showed that emulsion particle size of both kinds of vaccine were about 1.0 mm. Polydispesity index (PDI) value of ME vaccine was 0.036, and that of HS vaccine 0.279. ME vaccine showed better uniformity. The two kinds of vaccine had no significant difference in HI and IgG antibody level. Inflammatory factor IL-1b, IL-6, IL-17 and TNF-a gene expression levels of HS vaccine were up to 45, 7, 25 and 80 pg/mL, which were significantly higher than that of ME vaccine (the expression quantities were all less than 5 pg/mL). The white oil emulsion adjuvant with uniform size could induce enough immune protection, and cause milder inflammation.