采用氧化石墨烯(GO)辅助乙醇回流提取竹叶黄酮，通过部分析因?响应面法得到提取竹叶黄酮的最优条件，分析了提取机理. 结果表明，最优提取条件为温度82℃、时间86 min、乙醇浓度80%(?)、液固比17 mL/g，GO加入量为1.5 mg/g(干物料)，该条件下竹叶黄酮的提取率可达88.4%，比乙醇回流提取高约10.6%. GO能水解植物细胞壁，降低纤维素的结晶度和晶粒尺寸，使传质阻力减小，从而提高竹叶黄酮的提取率.

Graphene oxide (GO)-assisted ethanol reflux extraction (GERE) was used to extract flavonoids in bamboo leaves. The parameters of GERE were optimized by the fractional factorial?response surface method, and the extraction mechanism was analyzed. The results showed that the optimal conditions were ethanol 80%(?), ratio of liquid to solid 17 mL/g, addition GO 1.5 mg/g at 82℃ for 86 min. Under optimized conditions, the yield of total flavonoids was 88.4%, about 10.6% higher than ethanol reflux extraction (ERE). GO can lead to the destruction of the cell wall, the reduction of the crystallinity and the grain sizes of cellulose, which improved the extraction rate of bamboo leaves flavonoids. Thus, it was indicated that GERE method is an efficient, green, novel method for extraction of bamboo leaves flavonoids.